Inhibitory effect of co-culture of CEA-rV loaded dendritic cells and CIK cells on the growth of colon cancer xenografts in nude mice

Tian Hui¹Qi Yanchao¹Wang Yuandong²Yang Bo¹Lu Minying¹Shen Hongzhuo¹Pan Dongxiao¹

1. Department of Bioengineering, Affiliated Tumor Hospital of Guangzhou Medical College2. Department of Thoracic Surgery, Affiliated Tumor Hospital of Guangzhou Medical College

Abstract: Objective to investigate the load of CEA-rV dendritic cells and CIK cells after co-culture in nude mice in vivo specific anti-tumor effect. Methods Balb/c nude mice were inoculated with Lovo colon cancer cells subcutaneously on the nape of the neck, and treated with different effector cells (CIK, DC + CIK, CEA-rV + DC + CIK) for 6 days from the next day to observe the inhibitory effect on the growth of colon cancer. Results In vivo experiments in nude mice showed that CEA-rV + DC + CIK could significantly inhibit the growth of CEA-positive Lovo colon cancer cells, and its tumor inhibition rate could reach 51.2, which was higher than 25.1 in CIK group and 28.5 in DC + CIK group. Conclusion CEA-rV-loaded DC co-cultured with CIK cells has a specific anti-tumor effect on CEA-positive Lovo colon cancer in nude mice.

Key words:Dendritic cells;CIK cells;CEA-rV;Lovo colon cancer cells;

Fund assistance: Scientific Research Fund of Guangdong Provincial Science and Technology Department (No: 2006B35502013);

• Album: Medical and Health Technology
• Special Topic: Oncology
• Classification number: R735.35

CEA-rV load dendritic cells... inhibition of colon cancer xenografts growth_Tian Hui. pdf