PREPARATION AND DETERMINATION OF BIOLOGICAL ACTIVITY OF CD3AK CELLS IN CORD BLOOD: A REPORT OF 10 CLINICAL APPLICATIONS

Qi Yanchao, Wang Yuandong, Zhang Xiaomin

1. Department of Biological Engineering, Clinical Oncology Research Center of Guangzhou Medical College and Guangzhou Cancer Hospital 2. Department of Biological Engineering, Clinical Oncology Research Center of Guangzhou Medical College and Guangzhou Cancer Hospital 3. Department of Biological Engineering, Clinical Oncology Research Center of Guangzhou Medical College and Guangzhou Cancer Hospital 5100954.5100955.510095

Abstract: Objective To study the anti-tumor effect of CD3AK cells prepared from umbilical cord blood mononuclear cells, and to explore the immune indicators of the short-term efficacy of tumor biotherapy. Methods Cord blood mononuclear cells were isolated and IL-2 and IL-2 CD3Ab were used to induce LAK and CD₃The NK cell killing activity of peripheral blood mononuclear cells (PBMC) in tumor patients before and after treatment with CD3AK cells was measured. Results Umbilical cord blood LAK cells and cord blood CD3AK cells had the highest expansion times at d11 after culture, which were 18 times and 24 times respectively before culture, and their killing activity to K562 was 2.6 times and 3.2 times respectively before culture. After one course of CD3AK cell infusion, the NK killing activity of PBMC increased from 63% to 81%, with an average increase of 28%. Conclusion (1) Umbilical cord blood mononuclear cells are good precursors of LAK cells and CD3AK cells. (2) The number and killing ability of LAK and CD3AK cells peaked at d11 in culture, and the number and killing activity of CD3AK cells were significantly better than those of LAK cells. (3)CD3AK cell infusion can significantly improve the NK activity of PBMC in tumor patients. (4) Determination of NK activity of PBMC in tumor patients is expected to be a short-term therapeutic parameter of tumor biotherapy.

Key words:Cord blood;CD3AK cells;biological activity;

• Album: Medical and Health Technology
• Special Topic: Oncology
• Classification number: R73-36

Preparation of Cord Blood CD3AK Cells and Clinical Application Report of 10 Cases (English)_Qi Yanchao. pdf