Effect of PIK3CA overexpression on invasion of gastric cancer cells

Liu Jifang¹Li Wen²Qi Yanchao¹

1. Experimental Center of Cancer Hospital Affiliated to Guangzhou Medical College2. Department of Allergy, the Second Affiliated Hospital of Guangzhou Medical College

Abstract:Objective: To investigate the effect of PIK3CA overexpression on the invasion of gastric cancer cells. Methods: RT-PCR and Western blotting were used to detect the expression of PIK3CA mRNA and protein in HGC-27, BGC-823 and SGC-7901 of three different differentiated gastric cancer cells. The invasiveness of gastric cancer cells was detected by Transwell invasive chamber method, and the effect of PIK3CA expression level on the invasiveness of gastric cancer cells was analyzed. Results: The expression level of PIK3CA mRNA and protein was the highest in undifferentiated HGC-27 cells, and the cell invasion was the strongest (the number of transmembrane cells was 45.24±3.16), followed by poorly differentiated BGC-823 cells (the number of transmembrane cells was 22.28±1.65) and moderately differentiated SGC-7901 cells (the number of transmembrane cells was 14.42±1.02) (P<0.05). Conclusion: The higher the expression level of PIK3CA mRNA and protein, the stronger the cell invasion, and the latter decreased with the decrease of the former expression. PIK3CA overexpression may play an important role in the invasion of gastric cancer cells.

Key words:Gastric neoplasms;PIK3CA;Western blotting;invasion;

Funding:Project of Guangzhou Municipal Health Bureau (2009-YB-169);

Change to:10.16073/j.cn ki.cjcpt.2010.21.006

Album:Medical and Health Science and Technology

Topic:Oncology

Classification number:R735.2

Effect of PIK3CA overexpression on invasion of gastric cancer cells_Liu Jifang. pdf