Study on the sensitivity of human lung cancer A549 cells to CIK after immune editing by IFN-γ

Liu Jifang¹Liu Feng²Qi Yanchao¹Yang Bo¹Lu Minying¹Pan Dongxiao¹Shen Hongzhuo¹

1. Experimental Center of Cancer Hospital Affiliated to Guangzhou Medical College2. ECT Room, Affiliated Tumor Hospital of Guangzhou Medical College

Abstract:Objective: To investigate the reverse effect of IFN-γ on CIK killing sensitivity of human lung cancer A549 cells after immunoediting. Methods: RT-PCR and MTT were used to detect the effect of IFN-γ on the expression of MICA on the surface of A549 cells after immune editing and the change of the sensitivity of A549 cells to CIK killing. Results: After immunoediting, the expression of MICA on the surface of A549 cells was low, and the killing sensitivity of CIK cells was low. IFN-γ can significantly up-regulate the expression rate of MICA mRNA in A549 cells after editing, and improve the cytotoxic activity of CIK on A549 cells after editing. Conclusion: IFN-γ can enhance the expression of MICA mRNA on the surface of A549 cells and reverse the killing sensitivity of A549 cells to CIK cells after immunoediting.

Key words:IFN-γ;immune editing;reversal;CIK;

Funding:Project funded by Guangzhou Municipal Health Bureau (2009-YB-169);

Change to:10.13423/j.cn ki.cjcmi.005451

Album:Medical and Health Science and Technology

Topic:Oncology

Classification number:R734.2

IFN-γ Reverses Immune Editing Later People... Study on Cell Sensitivity to CIK Killing_Liu Jifang. pdf