Effect of Tongdu Xingshen Acupuncture on Apoptosis of Nerve Cells and Expression of Nerve Growth Factor Protein in Brain Tissue of Young Rats with Cerebral Palsy
2009年10月22日
Liu Zhenhuan1Pan Pei Guang1Qi Yanchao2Zhao Yong1firewood iron3Tang Chunzhi3Wang Qinyu3Yang Junjun3
1.Nanhai Obstetrics and Gynecology and Children's Hospital Affiliated to Guangzhou University of Chinese Medicine2.Guangzhou Cancer Hospital3.Guangzhou University of Chinese Medicine
Abstract:Objective To observe the effect of Tongdu Xingshen acupuncture on brain nerve cell apoptosis, nerve growth factor protein expression and limb function in young rats with cerebral palsy, and to analyze whether this effect is related to acupuncture time. Methods The experiment was completed in the clean laboratory of Experimental Animal Center and the laboratory of Acupuncture and Massage College of Guangzhou University of Chinese Medicine from August to October 2005. ① 109 7-day-old newborn SD pups were selected. After ligating the left common carotid artery, the rats were recovered for 2h, placed in a transparent closed container, and added into 37 ℃ constant temperature water at a rate of 1L/min to pass hypoxic gas (volume fraction 0.08 oxygen and 0.92 nitrogen). After 2.5h, the animals were taken out, and the survivors were kept warm for 1h for behavior measurement. Those who could not turn over, had abnormal balance or left-handed were regarded as successful models (72 rats); the remaining 24 rats were set as sham operation group: only the left common carotid artery was isolated without ligation and hypoxia treatment. Seventy-two rats with successful modeling were randomly divided into three groups: cerebral palsy rats acupuncture group I, cerebral palsy rats acupuncture group II, and model group, with 24 rats in each group. The rats were selected as Baiwill, affected side rate Gu Toujiao Sun, Neiguan, Quchi, Zusanli and Yongquan. Acupuncture group I of cerebral palsy young rats started acupuncture 24h after modeling, only acupuncture points on the limbs were added on the first 7 days, and acupuncture points on the head were added on the second day. Acupuncture was started on the 8th day after the model was made in the acupuncture group II of young rats with cerebral palsy, and the head and body needles were synchronized. Both groups were treated with acupuncture needles in Neiguan and Yongquan, without leaving needles, and then needle heads and Quchi and Zusanli. Baiwei, Ligu Toujiao Sun and Quchi and Zusanli are connected with G6805-Ⅱ electroacupuncture instrument, continuous wave, frequency 5~10Hz, duration 10min, once/d. Cerebral palsy rats acupuncture group Ⅰ continuous acupuncture 20d, cerebral palsy rats acupuncture group Ⅱ continuous acupuncture 13d. The acupuncture method in sham operation group was the same as that in group I of cerebral palsy rats. The model group only made models without any treatment. ③ On the 7th, 14th and 21st days after operation, the adhesive tape was stuck to the ventral sides of the two forepaws of rats, and the time taken to tear off the adhesive tape was recorded. ④ On the 21st day after operation, 10 rats were randomly selected from each group for the following tests: in situ end labeling and immunohistochemical methods were used to determine neuronal apoptosis and nerve growth factor expression in the frontal cortex and hippocampus of rats, and the number of apoptotic nerve cells and the number of nerve growth factor immune positive cells were counted by image analyzer under light microscope (× 400). The comparison of the rate was carried out by x2 test, and the multi-mean comparison between groups was carried out by analysis of variance (F-q examination). Results 109 rats were included, 13 rats were lost due to model failure, and no animals died in the sham operation group. 7d after modeling, cerebral palsy rats acupuncture group Ⅰ, cerebral palsy rats acupuncture group Ⅱ, model group died 2, 5, 3; 7 ~ 14d after modeling, the three groups died 2, 2, 4; 14 ~ 21d after modeling, the three groups died 0, 0, 2. ① Neuronal apoptosis in hippocampus and frontal lobe cortex of rats: The positive cells stained by in situ end labeling in hippocampus and frontal lobe cortex of rats in the two acupuncture treatment groups were significantly reduced, and the difference was significant compared with the model group (P<0.05). And the number of nerve cell apoptosis in the ten acupuncture I group of cerebral palsy rats was significantly lower than that in the ten acupuncture II group of cerebral palsy rats (P<0.01), the acupuncture group I of cerebral palsy rats was significantly more than or higher than that of the acupuncture group II of cerebral palsy rats (P<0.01). ③ On the 7th day after operation, the time taken to tear off the adhesive tape in the acupuncture group I of cerebral palsy rats was significantly shorter than that in the model group (P<0.01). On the 14th day after operation, the model group was significantly longer than the other three groups (P<0.05~0.01); On the 21st day after operation, the forelimb function of the two acupuncture groups and the model group were significantly improved, and the acupuncture group I of cerebral palsy rats was basically close to the normal level (compared with the sham operation group, the difference was not obvious, P>0.05), but the model group was still significantly longer than the acupuncture group I and the sham operation group of cerebral palsy rats (P<0.01). Conclusion Acupuncture can inhibit the apoptosis of nerve cells in the brain of cerebral palsy rats, enhance the expression of nerve growth factor in the brain tissue of cerebral palsy rats, improve limb function, and has a certain protective effect on brain tissue injury of cerebral palsy rats, and the earlier the intervention, the better the effect.
Key words:Cerebral palsy;Brain/acupuncture therapy;Apoptosis;Nerve growth factors;Tongdu Awakening Acupuncture;
Conference Name:2009 Shanghai · The 3rd Fuyang Forum and Fuyang School Theory and Clinical Application Training Course
Meeting time:2009-10-22
Venue:Shanghai, China
Album:Medical and Health Science and Technology
Topic:Traditional Chinese Medicine
Classification number:R245.3.
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