Comparison of Killing Effects of Cytokine-induced Killer Cells from Different Sources in Vitro

Zhou Minyi¹Qi Yanchao¹Huang Jianyun²

1. Cancer Institute, Affiliated Cancer Hospital of Guangzhou Medical College2. Department of Blood Transfusion, Guangzhou First People's Hospital, Guangdong Province

Abstract:Objective To compare the differences in the killing effect of cytokine-induced killer (CIK) cells derived from patients with advanced malignant tumors and those derived from healthy human CIK cells on lung cancer cell line A549, and to explore the mechanism of the differences, so as to provide a theoretical basis for the clinical application of healthy human CIK cells in the treatment of malignant tumors. Methods Peripheral blood mononuclear cells (PBMC) from patients with advanced malignant tumors and healthy people were extracted, and cytokines were added to induce CIK cells. LDH release method was used to detect the killing activity of PBMC and CIK cells from different sources on A549, flow cytometry was used to detect the cell phenotype and NK cell activated receptor (NKG2D) expression level, and ELISA method was used to detect the plasma MHC free class I related chain A molecule (sMICA) level. Results The expression of NKG2D receptor was higher in all subsets of CIK cells from healthy people, and the concentration of free MICA in plasma was lower than that of patients with advanced malignant tumor, which showed more effective anti-tumor effect. Conclusion Healthy human CIK cells have a good application prospect in the treatment of malignant tumors.

Key words:Cytokine-induced killer cells;Killing effect;Mechanisms;

Funding:Science and Technology Project in the Field of Social Development of Guangdong Province (No. [2010]1096-64);

Change to:10.13820/j.cn ki.gdyx.2012.16.061

Album:Medical and Health Science and Technology

Topic:Oncology

Classification number:R730.2

Comparison of in vitro killing effects of cytokine-induced killer cells from different sources.