Anti-Tumor Effect of Heat Shock Protein 70-Peptide Complexes on A-549 Cells

Wang Hongqi¹Hu Chenxia¹Hu Ling¹Shenweixi²Zhao Jian²Qi Yanchao²Han Xia¹Li Jianguo¹Zhang Zheng¹

1.Guangzhou University of Chinese Traditional Medicine2.Guangzhou Tumor Hospital

摘要：Objective: To investigate the anti-tumor immunity in vitro of heat shock protein 70-peptide complexes (HSP70-PC) from human lung cancer tissue. Methods: HSP70-PC was purified from lung tumor tissues and corresponding non-tumor lung samples with the methods of ADP-affinity chromatography, DEAE ion-exchange chromatography and Western-blot. The activation and proliferation of PBMC induced by different HSP70-PC and tumor cytotoxic reactivity to A549 cells in vitro were measured by the MTT cell proliferation assay. Results: The purified HSP70-PC had a very high purity found by SDS-PAGE and Western-blot. Human lymphocytes were sensitized efficiently by HSP70 preparation purified from lung cancer tissues and a definite cytotoxicity to A-549 cells was observed. There was significant difference with HSP70-PC purified from lung cancer, compared with the control group (P<0.001). Conclusion: High purity of HSP70-PC could be achieved from tumor tissues in this study. HSP70-PC purified from human tumor tissues can induce anti-tumor immunity in vitro mainly implemented by eliciting CTL immunity. Restore

基金资助：a grant from the Natural Science Foundation of Guangdong Province, China (No. 04010022).；

• Album: Medical and Health Technology
• Special Topic: Oncology
• Classification number: R734.2

Anti-Tumor Ef...n A-549 Cells_王洪琦.pdf