In vitro killing effect of DC vaccine loaded with two different antigen sensitization methods on Lovo cells

Liu Jifang Qi Yanchao Yang Bo Lu Minying Pan Dongxiao Shen Hongzhuo

The Affiliated Cancer Hospital of Guangzhou Medical College

Abstract:Objective To compare the effects of recombinant vaccinia virus loaded human carcinoembryonic antigen (CEA) gene transfection and Lovo cell lysate induction on the production of DC vaccine. Methods Immature DC were transfected by recombinant vaccinia virus loaded CEA gene and Lovo tumor cell lysate, respectively, to induce specific cytotoxic T lymphocytes (CTL). CTL was cultured in vitro and its activity was detected. MTT assay was used to detect the killing effect of CTL on Lovo cells. Results Both sensitized DC could induce the activation of CTL and secrete INF-γ, and the CTL induction rate of recombinant vaccinia virus transfected DC group was higher than that of cell lysate group. The specific killing activity of CTL induced by recombinant vaccinia virus transfected group was significantly higher than that of cell lysate group. Conclusion Two different antigen-sensitized DC vaccines can induce efficient and specific CTL tumor killing activity, and the use of recombinant vaccinia virus transfection CEA to DCs is significantly better than the antigen loading method of tumor cell lysate, which lays the foundation for the immunotherapy of colon cancer with DC vaccine.

Key words:Dendritic cells;Human carcinoembryonic antigen; Recombinant vaccinia virus;Cytotoxic T lymphocytes;

Album:Medical and Health Science and Technology

Topic:basic medicine

Classification number:R392

In vitro killing effect of D...Lovo cells loaded with two different antigen sensitization methods_Liu Jifang. pdf