NGF Enhances Experimental Immune Function in Mice

Qi Yanchao¹Liu Zhenhuan²firewood iron³

1. Affiliated Cancer Hospital of Guangzhou Medical College2. Nanhai Obstetrics and Gynecology and Children's Hospital Affiliated to Guangzhou University of Chinese Medicine3. College of Acupuncture and Massage, Guangzhou University of Chinese Medicine

Abstract:Objective To study the effect of nerve growth factor (NGF) injection on experimental immunosuppression in mice. Methods In an animal model of immunocompromised mice caused by cyclophosphamide (Cy80mg/kg, subcutaneous injection), the relevant immune indicators were determined by carbon particle clearance, one-way immunodiffusion, dinitrofluorobenzene-induced delayed type hypersensitivity, 3H-TdR participation and indirect immunolight. Results NGF injection [0.01, 0.1mg/(kg d)× 10d,im] could significantly increase the reduced K value, A value, serum IgG, ear swelling degree and ConA-induced T lymphocyte proliferation reaction value of mice. At the same time, it can increase the decreased L3T4 cells in peripheral blood of mice, thus increasing the decreased L3T4 /Lyt-2 cell ratio. Conclusion NGF injection has a significant enhancement effect on experimental immunodeficiency in mice, which provides a theoretical basis for the clinical application of tumor patients with nerve injury caused by radiotherapy and chemotherapy.

Key words:NGF;Cyclophosphamide;Immunoregulation;T lymphocyte subsets;mice;

Funding:National Natural Science Foundation of China (30740074); Natural Science Foundation of Guangdong Province (5002028);Guangzhou Municipal Health Bureau Integrated Traditional Chinese and Western Medicine Project (2005A040);

Album:Medical and Health Science and Technology

Topic:basic medicine

Classification number:R392

NGF Enhances Experimental Immune Function in Mice_Qi Yan Chao. pdf