In vitro proliferation and anti-tumor effects of CIK cells from umbilical cord blood

Yang BoLu MinyingPan DongxiaoShen HongzhuoQi Yanchao

Department of Bioengineering, Affiliated Tumor Hospital of Guangzhou Medical College

Abstract: Objective To study the proliferative activity of cord blood cytokine-induced killer cells (CIK cells) in vitro and their cytotoxicity to tumor cells, and to provide experimental basis for the application of CIK cells in tumor adoptive immunotherapy. Methods Mononuclear cells were obtained from umbilical cord blood by density gradient centrifugation, and CD₃A variety of CIK cells were prepared by monoclonal antibodies, recombinant human interleukin-2 (rhIL-2), recombinant human interleukin-1 (rhIL-1) and recombinant human interferon-γ(rhIFN-γ) as inducers. Lymphokine activated killer (LAK) cells induced by IL-2 alone and cord blood mononuclear cells (CBMNC) without cytokines were used as controls. Before and after culture, the cell morphology was observed by microscope, the change of cell phenotype was determined by flow cytometry, the cell proliferation level was determined by trypan blue exclusion method, and the killing activity of lung cancer cells was determined by tetramethylazolium (MTT) method. Results Through the combination of cytokines, a large number of highly active CIK cells could be induced. CIK cells began to proliferate on the 5th day of culture and reached a peak on the 14th day.₃⁺ CD₅₆⁺Cell-based. The proliferation peak of LAK cells appeared on the 7th day, and then the proliferation was not obvious. There was no obvious change in CBMNC phenotype, and the proliferation was not obvious. The in vitro killing activity of CIK cells against tumor cells was higher than that of LAK cells and CBMNC. Conclusion A large number of CIK cells can be successfully induced from umbilical cord blood under the combined action of cytokines in vitro. Cord blood CIK cells expand rapidly in vitro, have strong killing activity, and have better effect on tumor cells than traditional LAK cells, which provides a new direction for the adoptive immunotherapy of tumor.

Key words:fetal blood;Cytokine-induced killer cells;In vitro;Cell proliferation;Cytotoxicity, Immunity;

• Album: Medical and Health Technology
• Special Topic: Oncology
• Classification number: R730.5

In vitro proliferation and anti-tumor effect of CIK cells from umbilical cord blood