In vitro induction of specific T cell immunity by dendritic cells loaded with human carcinoembryonic antigen recombinant vaccinia virus

Zhong LiangxingQi YanchaoYang Bo

The Affiliated Cancer Hospital of Guangzhou Medical College

Abstract: Objective: To investigate whether human carcinoembryonic antigen (CEA)-specific cytotoxic T lymphocyte immunity can be induced by human carcinoembryonic antigen (CEA-rV) recombinant vaccinia virus loaded with cord blood-derived dendritic cells (DC) in vitro. Methods: DCs derived from cord blood monocytes with CEA-rV load were used to excite homologous T cells, and their proliferation effect on T cells and killing activity on CEA-secreting tumor cells were detected, and compared with T cells stimulated by DCs without CEA-rV load. Results: T cells activated by CEA-rV-loaded DC had specific killing effect on CEA-secreting tumor cells. Conclusion: CEA-rV loaded DC can induce CEA-specific T cell activity.

Key words:Vaccinia virus;Carcinoembryonic antigen;Dendritic cells (DCs);T lymphocytes;

• Album: Medical and Health Technology
• Topic: Basic Medicine
• Classification number: R392

Human carcinoembryonic antigen recombinant vaccinia virus load... Study on induction of specific T cell immunity_Zhong Liangxing.pdf