Effect of healthy human CIK cells infusion on cellular immune function in patients with malignant tumor

Huang Jianyun¹Li Yin²Zhou Minyi³Pan Dongxiao³Qi Yanchao³

1. Department of Blood Transfusion, Guangzhou First People's Hospital, Guangzhou Medical College2. Department of Thoracic Surgery, Henan Cancer Hospital3. Cancer Institute, Cancer Hospital, Guangzhou Medical College

Abstract:Objective: To study the changes of cellular immune function in peripheral blood of patients with malignant tumor before and after infusion of cytokine-induced killer cells (CIK cells) from healthy people. Methods: Peripheral blood mononuclear cells (PBMC) were isolated from healthy people, CIK cells were induced by rhIFN-γ, rhIL-2, rhIL-1β and CD3 monoclonal antibody (CD3mAb), and then returned to the patients. The killing activity of PBMC on K562 and the absolute count of T lymphocyte subsets in peripheral blood were compared before and after treatment with CIK cells in tumor patients. Results: The killing activity of PBMC and the absolute count of lymphocyte subsets in cancer patients before treatment were lower than those in healthy people. After treatment, the absolute count of NK cells was not significantly changed, but it was still lower than that in healthy people. Conclusion: CIK cell therapy can improve the anti-tumor ability of cancer patients.

Key words:healthy human CIK cells;Malignant tumor;Cellular immune function;

Funding:Guangdong Science and Technology Project (20100309);

Album:Medical and Health Science and Technology

Topic:Oncology

Classification number:R730.5

Effect of healthy human CIK cell infusion on cellular immune function in patients with malignant tumor